Effect of thoracic arthrodesis in prepubertal New Zealand white rabbits on cardio-pulmonary function
Federico Canavese1, Alain Dimeglio2, Davide Barbetta3, Bruno Pereira4, Sergio Fabbro5, Federica Bassini5, Bartolomeo Canavese6
1 Department of Pediatric Surgery, Universitys Hospital Estaing, 1 Place Lucie et Raymond Aubrac, 63003; Faculty of Medicine, University of Auvergne, 28 place Henri-Dunant BP 38 63001 Clermont-Ferrand, France
2 Faculty of Medicine, University of Montpellier, 2 Rue de l'Ecole de Medecine, 34000 Montpellier, France
3 Department of Life Sciences, University of Trieste, Animal Facility, Via Valerio 28, 34127 Trieste, Italy
4 Department of Research and Innovation (CRCI), Biostatistic Unit, University Hospital of Clermont Ferrand, Clermont Ferrand, France
5 Veterinary Clinic, Via Campos 192, 33030 Maiano (UD), Italy
6 University of Udine, Via Palladio 8, 33100 Udine, Italy
Department of Pediatric Surgery, University Hospital Estaing, 1 Place Lucie et Raymond Aubrac, 63003 Clermont Ferrand
Source of Support: None, Conflict of Interest: None of the authors of this paper have a financial or personal relationship with other people or organisations that could inappropriately influence or bias
the content of the paper.
Background: This experimental study was aimed at evaluating the type of cardiac and pulmonary involvement, in relation to changes of the thoracic spine and cage in prepubertal rabbits with nondeformed spine following dorsal arthrodesis. The hypothesis was that T1-T12 arthrodesis modified thoracic dimensions, but would not modify cardiopulmonary function once skeletal maturity was reached.
Materials and Methods: The study was conducted in 16 female New Zealand White (NZW) rabbits. Nine rabbits were subjected to T1-T12 dorsal arthrodesis while seven were sham-operated. Echocardiographic images were obtained at 12 months after surgery and parameters for 2-dimensional and M-mode echocardiographic variables were assessed. One week before echocardiographic examination, blood samples were withdrawn from the animals' central artery of the left ear to obtain blood gas values. One week after echocardiographic assessment, a thoracic CT scan was performed under general anesthesia. Chest depth (CD) and width (CW), thoracic kyphosis (ThK) and sternal length (StL) were measured; thoracic index (ThI), expressed as CD/CW ratio. All subjects were euthanized after the CT scan. Heart and lungs were subsequently removed to measure weight and volume.
Results: The values for 2-dimensional and M-mode echocardiographic variables were found to be uniformly and significantly higher, compared to those reported in anesthetized rabbits. CD, ThK, and StL were considerably lower in operated rabbits, as compared to the ones that were sham-operated. Similarly, the ThI was lower in operated rabbits than in sham-operated ones.
Conclusion: Irregularities in thoracic cage growth resulting from thoracic spine arthrodesis did not alter blood and echocardiographic parameters in NZW rabbits.