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MISCELLANEOUS
Year : 2006  |  Volume : 40  |  Issue : 4  |  Page : 264-266

Role of polymerase chain reaction in osteoarticular tuberculosis


1 Department of Orthopaedics, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India
2 Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India

Correspondence Address:
Atul Sareen
A 218 Meerabagh, Outer Ring Road, New Delhi 110087
India
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Source of Support: None, Conflict of Interest: None


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Background : Tuberculosis is a serious disease of global importance with a rising incidence worldwide. Available investigations like AFB stain, culture have low sensitivity and specificity, so there is need for a diagnostic test which is more sensitive and specific and less time consuming to provide final result. PCR is said to provide result within 36-48 hrs with high specificity and sensitivity. Method : Forty one cases showing clinicoradiological features of osteoarticular tuberculosis were studied with 20 cases taken in negative control group. Samples for PCR included synovial fluid and tissue Biopsy. PCR was used to amplify IS6110 genomic sequence of M. tuberculosis in the sample material to provide diagnosis. Results : The sensitivity of PCR was found to be 73.17%; and specificity 100%. The report of PCR was available within 36-48 hrs. Conclusion : By our study we conclude that PCR is a rapid highly sensitive and specific modality for detecting osteoarticular tuberculosis.


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